Product highlights
1.Superior stability with low pH;
2.Decreases viscosity quickly;
3.Enhances liquefaction efficacy;
4.Avoid formation of chromophore;
5.Eliminate non-fermentable sugars;
6.No need to add calcium under majority processing conditions;
7.Capable to handle various starch materials, such as tapioca, corn, wheat and rice.
Description
Thermostable Alpha-Amylase HT-320 is derived from strain of Bacillus licheniformis by submerge fermentation, extraction and refining process.
HT-320 is an endoamylase which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α -1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides. It can be applied without adding calcium ions during liquefaction process of production.
As a very robust alpha-amylase with superior thermal stability and wide pH range, HT-320 is widely used in starch sugar, ethanol, monosodium glutamate, beer, brewing, organic acid and antibiotics industry.
Product Characteristics
Declared Enzyme | Alpha-Amylase |
Systematic Name |
EC 3.2.1.1, 1, 4-alpha-D-glucan glucanohydrolase |
Activity | 40,000 U/ml (minimum) |
Appearance | Light to dark brown liquid |
Product pH | 5.5 to 7.0 |
Specific gravity | 1.15 to 1.25 g/ml |
Effect of pH
HT-320 is active with pH range 5.0~7.0, the optimal pH is 5.5~6.2.
Effect of Temperature
For interval liquefaction, the optimum temperature of HT-320 is above 90° C. It liquefies starch substrates promptly at 95 to 97° C and maintains highly active at 100° C. For consecutive liquefaction, HT-320 demonstrates exceptional thermal stability at temperatures from 105 to 110° C, maintaining its stability for 5 to 7 minutes, keep on liquefying rapidly, efficiently and completely.
Usage Guidelines
For industrial application of one-time spray liquefaction, first prepare 30~35% w/w starch slurry, adjust pH to 5.5~6.5 and then add this product, dosage should be 0.2~0.6kg/TDS. Liquefying at 105~110° C and maintain for 5~7 minutes, flash cooling down to around 97° C, keep liquefaction at temperature above 95° C for 90~120 minutes so as to reach required DE value.
Exact dosage of HT-320 depends on the starch substrate, liquefaction equipment and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
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